158 research outputs found
Implementing Roving Reference during the Fall Semester 2019 at Library West of the University of Florida's George Smathers Libraries
Library West of the University of Florida’s George Smathers Libraries is implementing a pilot roving reference librarian service in the Fall 2019 semester. Library West is the Humanities and Social Sciences library, and it is one of six libraries within the George Smathers Libraries System. It currently staffs the reference desk from 9:00 a.m. to 5:00 p.m., Monday through Thursday, and from 9:00 a.m. to 1:00 p.m. (in person) and 1:00 p.m. to 3:00 p.m. (by phone) on Friday (University of Florida, George Smathers Library, n.d.). The proposed new service is a useful update to traditional reference services. It encourages librarians to leave the comfort of the stationary desk to meet patrons where they are. It does not replace traditional reference services. Rather, it supplements them, hoping to catch patrons who would not normally approach the reference desk. By implementing roving reference tactics, librarians may be able to reach those who are not comfortable asking for help or who do not know where the reference desk is or how it functions. The new roving reference service would involve a reference librarian leaving the library with an iPad and stationing him- or herself in a key, high-traffic area, such as a dormitory lobby or the dining hall. This service seeks both to further library outreach and to provide reference in a new way
A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability
To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately 4,700 viable yeast deletion mutants and determined the fitness of the double mutants. Fifty-six DNA2-interacting genes were identified. Clustering analysis of genomic synthetic lethality profiles of each of 43 of the DNA2-interacting genes defines a network (consisting of 322 genes and 876 interactions) whose topology provides clues as to how replication proteins coordinate regulation and repair to protect genome integrity. The results also shed new light on the functions of the query gene DNA2, which, despite many years of study, remain controversial, especially its proposed role in Okazaki fragment processing and the nature of its in vivo substrates. Because of the multifunctional nature of virtually all proteins at the replication fork, the meaning of any single genetic interaction is inherently ambiguous. The multiplexing nature of the current studies, however, combined with follow-up supporting experiments, reveals most if not all of the unique pathways requiring Dna2p. These include not only Okazaki fragment processing and DNA repair but also chromatin dynamics
Students’ Perception of Embedding Cultural Diversity Content into Early Childhood Courses
This study examined the incorporation of specific content framed with a diversity and equity lens into two existing early childhood courses. The purpose was to investigate how embedding the diversity and equity content impacted student perceptions and supported their cultural competence. Pre- and post-survey results (N = 50) show student awareness of biases and beliefs were positively influenced. Implications are examined related to course content and activities that could enhance students’ competencies and understanding to work with colleagues, children and families in diverse, inclusive settings
An interactional network of genes involved in chitin synthesis in Saccharomyces cerevisiae
BACKGROUND: In S. cerevisiae the β-1,4-linked N-acetylglucosamine polymer, chitin, is synthesized by a family of 3 specialized but interacting chitin synthases encoded by CHS1, CHS2 and CHS3. Chs2p makes chitin in the primary septum, while Chs3p makes chitin in the lateral cell wall and in the bud neck, and can partially compensate for the lack of Chs2p. Chs3p requires a pathway of Bni4p, Chs4p, Chs5p, Chs6p and Chs7p for its localization and activity. Chs1p is thought to have a septum repair function after cell separation. To further explore interactions in the chitin synthase family and to find processes buffering chitin synthesis, we compiled a genetic interaction network of genes showing synthetic interactions with CHS1, CHS3 and genes involved in Chs3p localization and function and made a phenotypic analysis of their mutants. RESULTS: Using deletion mutants in CHS1, CHS3, CHS4, CHS5, CHS6, CHS7 and BNI4 in a synthetic genetic array analysis we assembled a network of 316 interactions among 163 genes. The interaction network with CHS3, CHS4, CHS5, CHS6, CHS7 or BNI4 forms a dense neighborhood, with many genes functioning in cell wall assembly or polarized secretion. Chitin levels were altered in 54 of the mutants in individually deleted genes, indicating a functional relationship between them and chitin synthesis. 32 of these mutants triggered the chitin stress response, with elevated chitin levels and a dependence on CHS3. A large fraction of the CHS1-interaction set was distinct from that of the CHS3 network, indicating broad roles for Chs1p in buffering both Chs2p function and more global cell wall robustness. CONCLUSION: Based on their interaction patterns and chitin levels we group interacting mutants into functional categories. Genes interacting with CHS3 are involved in the amelioration of cell wall defects and in septum or bud neck chitin synthesis, and we newly assign a number of genes to these functions. Our genetic analysis of genes not interacting with CHS3 indicate expanded roles for Chs4p, Chs5p and Chs6p in secretory protein trafficking and of Bni4p in bud neck organization
The Vehicle, Spring 1992
Contents
POEMS
Makin\u27 Mudpies Nancy James page 6
Obscurity Kim Frost page 7
The Plea for a Pink One Victoria Bennett page 8
Mom\u27s Loving Push Amy Boone page 10
Through a Frog Laura Durnell page 12
Cold Snap A.L. Gallion page 12
Dimensity Anthony Smith page 13
Cold War Anthony Smith page 14
Get A Spoon Sheila Taylor page 15
Explore K. Thorsson page 16
FICTION
The Proofreader Jenny L. Shields page 18
Ba, Ba, Black Sheep Victoria Bennett page 22
Eat My Words Sheila Taylor page 27
BIOGRAPHIES page 30
all photography by Dan Kooncehttps://thekeep.eiu.edu/vehicle/1059/thumbnail.jp
Motifs, themes and thematic maps of an integrated Saccharomyces cerevisiae interaction network
BACKGROUND: Large-scale studies have revealed networks of various biological interaction types, such as protein-protein interaction, genetic interaction, transcriptional regulation, sequence homology, and expression correlation. Recurring patterns of interconnection, or 'network motifs', have revealed biological insights for networks containing either one or two types of interaction. RESULTS: To study more complex relationships involving multiple biological interaction types, we assembled an integrated Saccharomyces cerevisiae network in which nodes represent genes (or their protein products) and differently colored links represent the aforementioned five biological interaction types. We examined three- and four-node interconnection patterns containing multiple interaction types and found many enriched multi-color network motifs. Furthermore, we showed that most of the motifs form 'network themes' – classes of higher-order recurring interconnection patterns that encompass multiple occurrences of network motifs. Network themes can be tied to specific biological phenomena and may represent more fundamental network design principles. Examples of network themes include a pair of protein complexes with many inter-complex genetic interactions – the 'compensatory complexes' theme. Thematic maps – networks rendered in terms of such themes – can simplify an otherwise confusing tangle of biological relationships. We show this by mapping the S. cerevisiae network in terms of two specific network themes. CONCLUSION: Significantly enriched motifs in an integrated S. cerevisiae interaction network are often signatures of network themes, higher-order network structures that correspond to biological phenomena. Representing networks in terms of network themes provides a useful simplification of complex biological relationships
Histone modifications influence mediator interactions with chromatin
The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. Genome wide localization studies have demonstrated that Mediator occupancy not only correlates with high levels of transcription, but that the complex also is present at transcriptionally silenced locations. We provide evidence that Mediator localization is guided by an interaction with histone tails, and that this interaction is regulated by their post-translational modifications. A quantitative, high-density genetic interaction map revealed links between Mediator components and factors affecting chromatin structure, especially histone deacetylases. Peptide binding assays demonstrated that pure wild-type Mediator forms stable complexes with the tails of Histone H3 and H4. These binding assays also showed Mediator—histone H4 peptide interactions are specifically inhibited by acetylation of the histone H4 lysine 16, a residue critical in transcriptional silencing. Finally, these findings were validated by tiling array analysis that revealed a broad correlation between Mediator and nucleosome occupancy in vivo, but a negative correlation between Mediator and nucleosomes acetylated at histone H4 lysine 16. Our studies show that chromatin structure and the acetylation state of histones are intimately connected to Mediator localization
The Vehicle, Spring 1984
Vol. 25, No. 2
Table of Contents
The OpeningKathy Fordpage 3
PaintThomas B. Waltrippage 3
PhotographLisa Fresepage 4
City as Castigating AnimalJerry McAnultypage 5
LimitsMichelle Mitchellpage 6
In SilenceKeila Tooleypage 6
PhotographLisa Fresepage 7
Indian CreekJerry McAnultypage 8
The Right Idea about RainMichelle Mitchellpage 8
Van Gogh\u27s Young Male PeasantKeila Tooleypage 9
AbigailMaggie Kennedypage 9
CleaningJennifer K. Soulepage 10
Heat WaveJerry McAnultypage 11
VigilJanet Gracepage 11
Hatred of ClownsMichelle Mitchellpage 12
Trees in the YardKeila Tooleypage 13
Lost on Back RoadsToby Mirabellipage 13
The Death of My Father\u27s HandsGina Grillopage 14
Soft LandingMaggie Kennedypage 14
Incident in the Generic AisleMaggie Kennedypage 15
Happy Well-adjusted Individuals, ConsideringJennifer K. Soulepage 16
Old ManGina Grillopage 17
It Doesn\u27t MatterTed Boonepage 18
A Love Poem for Charles DonaldCathy Moepage 18
David Willey at Whitman WharfSue Oiler Millerpage 19
I Am the ArtsBrenda Renee Drummerpage 20
Mothers and DaughtersJennifer K. Soulepage 22
ScratchesMichelle Mitchellpage 26
SedativeStacey Flanniganpage 26
Calloused HandJanet Gracepage 27
Cool CatStacey Flanniganpage 27
For My History Teacher, Miss WilhelmCathy Moepage 28
Short Poem for Chris\u27s EyesBob Zordanipage 28
MissingMichelle Mitchellpage 29
Over ThereGraham Lewispage 30
Strange BedfellowsMaggie Kennedypage 31
Retired PlumberMichelle Mitchellpage 32
Rumors of War DeadKathy Fordpage 33
Night LightsKeila Tooleypage 33
Fish on the Train TracksJerry McAnultypage 34
DaddyKeily Tooleypage 34
A Message to the MessengerAmy Eadespage 35
Dancing AloneKathy Bowerpage 36
HandsMaggie Kennedypage 36
The Key to the Ladies\u27s RoomMichelle Mitchellpage 37
Crashing the BlueStacey Flanniganpage 37
The Cradle will FallMichelle Mitchellpate 38
One IslandKeila Tooleypage 38
Black Walnut PaintSue Oiler Millerpage 39
SerendipityGraham Lewispage 40
Cover ArtLisa Fresehttps://thekeep.eiu.edu/vehicle/1045/thumbnail.jp
Dr. Bobbie Bailey & Family Performance Center Anniversary Celebration
The School of Music is proud to welcome back to campus several of our esteemed alumni for a special recital as part of the Bailey Performance Center 10th anniversary celebration! The School of Music celebrates the opening of the Bailey Performance Center with featured performances by the KSU Wind Ensemble Brass and Percussion, Symphony Orchestra, Chamber Singers, University Chorale and Chamber Singers Alumni Choir, along with pianist Robert Henry, soprano Jana Young, and more!https://digitalcommons.kennesaw.edu/musicprograms/1969/thumbnail.jp
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